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Comparative Study
. 1979 Feb 26;60(1):85-9.
doi: 10.1007/BF00495731.

Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased

L Bell, L Williams
Comparative Study

Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased

L Bell et al. Histochemistry. .

Abstract

Alkaline phosphatase activity has been investigated by histochemical methods in normal and diseased human large intestine. The tissues were constantly maintained at 4 degrees C or below. Specimens were either frozen in liquid nitrogen, freeze-dried and embedded in glycol methacrylate for sectioning at 2 mu, or, fixed in ice-cold formol-calcium for frozen sectioning at 10 mu. The simultaneous coupling azo dye method using the substrates sodium alpha-naphthyl phosphate and Naphthol AS-BI phosphate, resulted in the demonstration of alkaline phosphatase activity in the surface epithelial cells, and the middle and upper crypts, of normal and transitional mucosa.

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