Transformation of arachidonic acid by rabbit polymorphonuclear leukocytes. Formation of a novel dihydroxyeicosatetraenoic acid

Abstract

A new metabolite of arachidonic acid, 5-D-(S),12-D-(R)-dihydroxy-6,8,10,14-eicosatetraenoic acid, was found upon incubation of the fatty acid with a suspension of rabbit peritoneal polymorphonuclear leukocytes collected 4 h after injection of glycogen into the peritoneal cavity. The yield of the dihydroxy acid was 0.5 to 2%. The compound possesses three conjugated double bonds and was found to be stereochemically pure at C-5 and C-12. Incubation of the cells with 8,11,14-eicosatrienoic acid did not lead to the formation of the analogous triunsaturated dihydroxy acid.