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. 1968 Jul 5;161(3836):57-9.
doi: 10.1126/science.161.3836.57.

Cyclic 3',5'-adenosine monophosphate phosphodiesterase produced by the slime mold Dictyostelium discoideum

Y Y Chang

Cyclic 3',5'-adenosine monophosphate phosphodiesterase produced by the slime mold Dictyostelium discoideum

Y Y Chang. Science. .

Abstract

An enzyme that hydrolyzes cyclic ',5'-adenosine monophosphate to 5'-adenosine monophosphate was found in the culture medium of the cellular slime mold Dictyostelium discoidum. The enzymatic activity shows a pH optimum of 7.5, and magnesium is required for maximum activity. The enzyme is not inhibited by caffeine. It has a Michaelis-Menten constant of 2 x 10(-3)M and its molecular weight is around 300,000.

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