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. 1968 Nov;2(11):1346-52.
doi: 10.1128/JVI.2.11.1346-1352.1968.

Characterization of intracellular ribonucleic acid specific for the murine sarcoma-leukemia virus complex

N Biswal, M B Grizzard, R M McCombs, M Benyesh-Melnick

Characterization of intracellular ribonucleic acid specific for the murine sarcoma-leukemia virus complex

N Biswal et al. J Virol. 1968 Nov.

Abstract

Virus particles were continuously produced by a cell line (78A1) of rat embryo fibroblasts that had been transformed by the murine sarcoma-leukemia virus complex. Since most of the mature virions were found in the extracellular fluid and were not cell-associated, a measurable quantity of viral ribonucleic acid (RNA) could not be extracted from these cells. Cycloheximide, a protein inhibitor, was successfully used to accumulate viral RNA within the cells. This ribonuclease-sensitive RNA, with a sedimentation coefficient of 71S, had the same base composition as the high molecular weight RNA (S(20,w) = 71) isolated from purified virions released by the transformed cells.

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References

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