Techniques utilizing real time stereo scanning electron microscopy in the microdissection of biological tissues

Abstract

The use of the scanning electron microscope (SEM) for the microdissection of biological tissue is described. The dissections were accomplished by the use of electrolytically pointed needles that were positioned in a simply constructed holder. The operations were viewed using real time stereo SEM, and recorded on videotape. Various methods for photographing the videotape recordings are outlined.