Identification of adenosine-3',5'-monophosphate as the bacterial attractant for myxamoebae of Dictyostelium discoideum

Abstract

Adenosine-3',5'-cyclic monophosphate was shown to be the compound found in Escherichia coli responsible for the attraction of the amoebae of the cellular slime mold Dictyostelium discoideum. A number of other nucleotides were tested and the following were active: tubercidin-3',5'-cyclic monophosphate, N(6)-2'-O-dibutyryl-adenosine-3',5'-cyclic monophosphate, 5'-methylene adenosine-3',5'-cyclic monophosphonate, guanosine-3',5'-cyclic monophosphate, uridine-3',5'-cyclic monophosphate, cytidine-3',5'-cyclic monophosphate, inosine-3',5'-cyclic monophosphate, and thymidine-3',5'-cyclic monophosphate. They were less active than adenosine-3',5'-cyclic monophosphate. It is suggested that cyclic adenosine monophosphate secreted by the bacteria is used by the amoebae as a means of sensing and orienting towards food.