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. 1969 Aug;114(1):49-56.
doi: 10.1042/bj1140049.

Purification of a trifunctional enzyme, catalysing three steps of the histidine pathway, from Neurospora crassa

A C Minson, E H Creaser

Purification of a trifunctional enzyme, catalysing three steps of the histidine pathway, from Neurospora crassa

A C Minson et al. Biochem J. 1969 Aug.

Abstract

1. A procedure is described for the purification of an enzyme from Neurospora crassa that has three catalytic functions. These are 1-N-(5'-phosphoribosyl)-ATP pyrophosphohydrolase, 1-N-(5'-phosphoribosyl)-AMP cyclohydrolase and histidinol dehydrogenase (l-histidinol-NAD oxidoreductase, EC 1.1.1.23), and are responsible for the catalysis of reactions 2, 3 and 10 in the histidine pathway. The ratio of these three catalytic activities remains approximately the same throughout the purification procedure. Evidence is presented that the purified preparations contain a single protein exhibiting association-dissociation equilibria.

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References

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