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. 1969 Dec;115(5):969-76.
doi: 10.1042/bj1150969.

The metabolism of D-glucarate by Pseudomonas acidovorans

R Jeffcoat, H Hassall, S Dagley

The metabolism of D-glucarate by Pseudomonas acidovorans

R Jeffcoat et al. Biochem J. 1969 Dec.

Abstract

1. Dehydratases that converted d-glucarate into 4-deoxy-5-oxoglucarate were partially purified from Klebsiella aerogenes and Pseudomonas acidovorans. 2. When d-glucarate was metabolized to 2,5-dioxovalerate it appeared that water and carbon dioxide were removed from 4-deoxy-5-oxoglucarate in one enzymic step: 4,5-dihydroxy-2-oxovalerate was not an intermediate in this reaction. 3. A method for the enzymic determination of d-glucarate is described.

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