Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 May;117(5):853-9.
doi: 10.1042/bj1170853.

Rates of aminoacyl-transfer-ribonucleic acid synthesis in vivo and in vitro by bean leaves

T C Hall, K L Tao

Rates of aminoacyl-transfer-ribonucleic acid synthesis in vivo and in vitro by bean leaves

T C Hall et al. Biochem J. 1970 May.

Abstract

1. A procedure for measuring rates of aminoacyl-tRNA synthesis in vitro and in intact leaves is presented. 2. Leaf discs showed rates close to those of intact leaves. 3. Cell-free preparations showed similar rates when assayed by pyrophosphate exchange, but actual aminoacyl-tRNA formation rates appeared to be much lower. Evidence is presented that dilution of supplied labelled amino acids was a major factor causing the low apparent rates. 4. Attempts to strip endogenous amino acids from plant tRNA resulted in low acceptor capability of the tRNA.

PubMed Disclaimer

References

    1. Biochem J. 1965 Sep;96(3):616-25 - PubMed
    1. Biochemistry. 1968 Mar;7(3):1100-5 - PubMed
    1. Biochim Biophys Acta. 1969 Feb 18;174(2):513-24 - PubMed
    1. Biochemistry. 1969 Jan;8(1):295-303 - PubMed
    1. J Virol. 1969 Jan;3(1):89-91 - PubMed