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. 1970 Jul;20(1):78-84.
doi: 10.1128/am.20.1.78-84.1970.

Characterization of clostridia by gas chromatography differentiation of species by trimethylsilyl derivatives of whole-cell hydrolysates

D C Farshy, C W Moss

Characterization of clostridia by gas chromatography differentiation of species by trimethylsilyl derivatives of whole-cell hydrolysates

D C Farshy et al. Appl Microbiol. 1970 Jul.

Abstract

Trimethylsilyl (TMS) derivatives prepared from whole-cell hydrolysates of 36 strains, representing 10 species of Clostridium were examined by gas-liquid chromatography (GLC). The TMS profile of each species contained a group of peaks which characterized the species. Variation among strains within a species was much lower than variation between species. Some of the closely related clostridia could be differentiated by comparing their TMS profiles. Strains of Clostridium botulinum were distinguished from C. sporogenes on the basis of the ratio of two GLC peaks which corresponded to arabinose and glucose. A peak with a retention time identical to that of mannose was present in all C. bifermentans strains but was absent in those of C. sordellii.

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References

    1. J Hyg (Lond). 1953 Mar;51(1):75-101 - PubMed
    1. Can J Microbiol. 1969 Dec;15(12):1433-47 - PubMed
    1. Bacteriol Rev. 1938 Jun;2(1):47-97 - PubMed
    1. J Chromatogr. 1969 Jun 3;42(1):108-11 - PubMed
    1. J Pathol Bacteriol. 1963 Jan;85:41-9 - PubMed

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