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. 1970 Sep;67(1):148-55.
doi: 10.1073/pnas.67.1.148.

Identification of proinsulin and C-peptide in human serum by a specific immunoassay

F Melani, A H Rubenstein, P E Oyer, D F Steiner

Identification of proinsulin and C-peptide in human serum by a specific immunoassay

F Melani et al. Proc Natl Acad Sci U S A. 1970 Sep.

Abstract

An immunoassay for the measurement of human proinsulin and C-peptide is described. By a combination of this system and an insulin immunoassay, proinsulin, insulin, and C-peptide were identified in acid-ethanol extracts of serum after gel filtration. In keeping with the results of biosynthetic studies, insulin and C-peptide were found to be present in equimolar amounts. The absolute values of serum proinsulin determined independently in the two assays corresponded closely.

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References

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