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. 1970 Sep;67(1):305-12.
doi: 10.1073/pnas.67.1.305.

A protein binding assay for adenosine 3':5'-cyclic monophosphate

A G Gilman

A protein binding assay for adenosine 3':5'-cyclic monophosphate

A G Gilman. Proc Natl Acad Sci U S A. 1970 Sep.

Abstract

A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.

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