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. 1967 Jun;1(3):472-7.
doi: 10.1128/JVI.1.3.472-477.1967.

Concentration of enteroviruses on membrane filters

C Wallis, J L Melnick

Concentration of enteroviruses on membrane filters

C Wallis et al. J Virol. 1967 Jun.

Abstract

Enteroviruses can be made to adsorb or to pass through membrane filters by manipulation of the suspending medium. Salts facilitate virus adsorption, but membrane-coating components (MCC) interfere. Because cells release MCC into the culture medium during viral growth, MCC must be removed before virus can be adsorbed to membranes. Adsorbed virus can be eluted with diluents containing MCC (cell extracts or serum) or agents that reduce surface tension (sodium lauryl sulfate). By membrane adsorption and elution, enteroviruses can be readily concentrated and quantitatively recovered from crude virus harvests.

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References

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