doi: 10.1073/pnas.66.1.146.
Binding of adenosine 3':5'-cyclic phosphate to G factor of Escherichia coli, and its effects on GTPase, RNase V, and protein synthesis
- PMID: 4320462
- PMCID: PMC286100
- DOI: 10.1073/pnas.66.1.146
Item in Clipboard
Binding of adenosine 3':5'-cyclic phosphate to G factor of Escherichia coli, and its effects on GTPase, RNase V, and protein synthesis
Proc Natl Acad Sci U S A.
1970 May.
Abstract
Unique among adenine nucleotides tested by filter binding assays, 3':5'-cyclic AMP binds to the G translocation factor. Binding is dependent on the presence of GTP, and is inhibited by GDP, by the analog 5'-beta,gamma-methylene GTP, and by the antibiotic fusidic acid. The cAMP seems to be released during the ribosome-dependent translocation of charged tRNA catalyzed by G factor. Bound cAMP inhibits GTPase and ribosome-associated degradation of messenger RNA, but does not inhibit protein synthesis. cAMP might thereby regulate the ratio of productive to degradative transits of ribosomes on messenger RNA, and this may account for some part of its profound effect on levels of specific bacterial messenger RNA species.
Similar articles
-
Ribonuclease V of escherichia coli. I. Dependence on ribosomes and translocation.Proc Natl Acad Sci U S A. 1969 Oct;64(2):693-700. doi: 10.1073/pnas.64.2.693. Proc Natl Acad Sci U S A. 1969. PMID: 4901707 Free PMC article.
-
Role of guanine nucleotides in protein synthesis. Elongation factor G and guanosine 5'-triphosphate,3'-diphosphate.Proc Natl Acad Sci U S A. 1973 Nov;70(11):3250-4. doi: 10.1073/pnas.70.11.3250. Proc Natl Acad Sci U S A. 1973. PMID: 4594040 Free PMC article.
-
Ribosome degradation and the degradation products in starved Escherichia coli. I. Comparison of the degradation rate and of the nucleotide pool between Escherichia coli B and Q-13 strains in phosphate deficiency.Biochim Biophys Acta. 1970 Jan 21;199(1):159-65. Biochim Biophys Acta. 1970. PMID: 4905130 No abstract available.
-
Regulation of bacterial growth.Science. 1974 Jun 7;184(4141):1043-50. doi: 10.1126/science.184.4141.1043. Science. 1974. PMID: 4620041 Review.
-
The 2',3'-cAMP-adenosine pathway.Am J Physiol Renal Physiol. 2011 Dec;301(6):F1160-7. doi: 10.1152/ajprenal.00450.2011. Epub 2011 Sep 21. Am J Physiol Renal Physiol. 2011. PMID: 21937608 Free PMC article. Review.
Cited by
-
Purification and identification of the fruiting-inducing substances in Coprinus macrorhizus.J Bacteriol. 1973 Mar;113(3):1240-8. doi: 10.1128/jb.113.3.1240-1248.1973. J Bacteriol. 1973. PMID: 4347967 Free PMC article.
-
A protein binding assay for adenosine 3':5'-cyclic monophosphate.Proc Natl Acad Sci U S A. 1970 Sep;67(1):305-12. doi: 10.1073/pnas.67.1.305. Proc Natl Acad Sci U S A. 1970. PMID: 4318781 Free PMC article.
-
Cyclic AMP receptor protein of E. coli: its role in the synthesis of inducible enzymes.Proc Natl Acad Sci U S A. 1970 Jun;66(2):480-7. doi: 10.1073/pnas.66.2.480. Proc Natl Acad Sci U S A. 1970. PMID: 4317918 Free PMC article.
-
RNA-directed cell-free synthesis of the galactose enzymes of Escherichia coli.Mol Gen Genet. 1973 Aug 28;124(4):329-44. doi: 10.1007/BF00267662. Mol Gen Genet. 1973. PMID: 4355744 No abstract available.
-
Adenosine 3':5'-cyclic monophosphate as a regulator of bacterial transformation.Proc Natl Acad Sci U S A. 1973 Feb;70(2):471-4. doi: 10.1073/pnas.70.2.471. Proc Natl Acad Sci U S A. 1973. PMID: 4346890 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
