Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Oct;23(7):594-8.
doi: 10.1136/jcp.23.7.594.

A fluorimetric and enzymatic method for the estimation of serum total bile acids

G M Murphy, B H Billing, D N Baron

A fluorimetric and enzymatic method for the estimation of serum total bile acids

G M Murphy et al. J Clin Pathol. 1970 Oct.

Abstract

The enzymatic technique using 3alpha-hydroxysteroid dehydrogenase for determining bile acids in blood has been modified by measuring the reduced nicotinamide adenine dinucleotide fluorimetrically. The increased sensitivity attained enables the concentration of total bile acids in serum to be estimated using 3 ml for normal subjects and 1 ml for jaundiced patients. The range of normal values in serum was found to be 0-4.7 mumol/litre for males and 1.0-8.2 mumol/litre for females.

PubMed Disclaimer

References

    1. J Lipid Res. 1966 Jul;7(4):579-81 - PubMed
    1. Nature. 1967 Jan 28;213(5074):404-5 - PubMed
    1. Can J Biochem. 1968 Apr;46(4):390-2 - PubMed
    1. J Clin Pathol. 1969 Mar;22(2):222-5 - PubMed
    1. Clin Chim Acta. 1969 May;24(2):253-9 - PubMed

LinkOut - more resources