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. 1971 Apr;21(4):703-9.
doi: 10.1128/am.21.4.703-709.1971.

Concentration and purification of viruses by adsorption to and elution from insoluble polyelectrolytes

C Wallis, J L Melnick, J E Fields

Concentration and purification of viruses by adsorption to and elution from insoluble polyelectrolytes

C Wallis et al. Appl Microbiol. 1971 Apr.

Abstract

Acid-resistant, nonenveloped viruses belonging to the enterovirus, reovirus, and adenovirus groups were readily concentrated on PE60, an insoluble cross-linked polyelectrolyte based on isobutylene maleic anhydride. Hydrolysis of PE60 by NaOH increased its capacity to adsorb viruses. Hydrogen ion levels played an important role in virus concentration; optimal pH levels for maximal virus adsorption were between pH 3.0 and 4.5. Undiluted virus was easily concentrated from large volumes on PE60, and the adsorbed virus was readily eluted at pH 8 to 9.

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References

    1. J Bacteriol. 1966 Jul;92(1):155-60 - PubMed
    1. Am J Epidemiol. 1967 May;85(3):459-68 - PubMed
    1. Acta Virol. 1968 Mar;12(2):185 - PubMed
    1. Appl Microbiol. 1969 Dec;18(6):1007-14 - PubMed
    1. Bull World Health Organ. 1970;42(2):291-6 - PubMed

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