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. 1971 Jun;20(6):1061-5.

A simple method for coupling proteins to insoluble polysaccharides

C J Sanderson, D V Wilson

A simple method for coupling proteins to insoluble polysaccharides

C J Sanderson et al. Immunology. 1971 Jun.

Abstract

Proteins have been coupled to insoluble polysaccharides following periodate oxidation to produce reactive aldehyde groups which will react with side chain amino groups of proteins. The coupling is stabilized and unreacted aldehydes blocked, by reduction with sodium borohydride.

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References

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    1. Proc Natl Acad Sci U S A. 1969 Jun;63(2):450-7 - PubMed
    1. Nature. 1970 Jan 10;225(5228):189-90 - PubMed
    1. Adv Carbohydr Chem. 1961;16:105-58 - PubMed

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