Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 Jul;8(1):66-73.
doi: 10.1128/JVI.8.1.66-73.1971.

Transcription of the influenza ribonucleic acid genome by a virion polymerase. I. Optimal conditions for in vitro activity of the ribonucleic acid-dependent ribonucleic acid polymerase

D H Bishop, J F Obijeski, R W Simpson

Transcription of the influenza ribonucleic acid genome by a virion polymerase. I. Optimal conditions for in vitro activity of the ribonucleic acid-dependent ribonucleic acid polymerase

D H Bishop et al. J Virol. 1971 Jul.

Abstract

In vitro reaction conditions have been determined for the maximal synthesis of product ribonucleic acid by the influenza (WSN) virion ribonucleic acid polymerase. The reaction requires the presence of all four triphosphates, Mg(2+) and Mn(2+) ions, monovalent cations, nonionic detergent, and ribonucleoside triphosphates at concentrations above certain threshold values. The optimum pH for the reaction is around 8.0 to 8.2 and the kinetics of product synthesis are linear through at least 6 hr when incubated at 31 to 33 C.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1968 Feb 26;155(2):609-10 - PubMed
    1. J Gen Virol. 1967 Oct;1(4):479-86 - PubMed
    1. Virology. 1968 Feb;34(2):385-8 - PubMed
    1. Virology. 1968 May;35(1):41-9 - PubMed
    1. J Gen Virol. 1969 Sep;5(2):283-90 - PubMed

MeSH terms

LinkOut - more resources