doi: 10.1128/JVI.7.4.486-490.1971.
Complete transcription by the transcriptase of vesicular stomatitis virus
- PMID: 4329565
- PMCID: PMC356148
- DOI: 10.1128/JVI.7.4.486-490.1971
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Complete transcription by the transcriptase of vesicular stomatitis virus
J Virol.
1971 Apr.
Abstract
Vesicular stomatitis virus (VSV) transcriptase completely transcribes the VSV genome into messenger-size ribonucleic acid (RNA) species. The replicating complexes of template and product RNA, generated in vitro by VSV transcriptase, exhibit a mass parity between product and template molecules. Purified product RNA, after annealing to small amounts of viral RNA, rendered 92% of the viral RNA ribonuclease-resistant. It is calculated that, under optimal conditions, 60 min is required before the VSV genome is completely transcribed.
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