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. 1971 Jan;121(2):241-4.
doi: 10.1042/bj1210241.

A radiochemical renin assay

F A Mendelsohn, C I Johnston

A radiochemical renin assay

F A Mendelsohn et al. Biochem J. 1971 Jan.

Abstract

1. A synthetic 3-([(14)C]valine)-labelled tetradecapeptide renin substrate was used to measure renin concentration. Renin liberated (14)C-labelled angiotensin I, which was separated from the labelled substrate by paper chromatography. The conversion of substrate into angiotensin I was quantitated by liquid-scintillation counting of radioactivity. 2. The rate of conversion of the substrate into angiotensin I was shown to be linearly related to renin concentration and time under suitable conditions. Angiotensin generation measured in this system agrees well with that measured by bioassay. 3. It is suggested that the use of a pure substrate offers advantages that include the standardization of current units of renin measurement.

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