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. 1971 Aug;68(8):1796-800.
doi: 10.1073/pnas.68.8.1796.

Inhibition by siomycin and thiostrepton of both aminoacyl-tRNA and factor G binding to ribosomes

J Modolell, B Cabrer, A Parmeggiani, D Vazquez

Inhibition by siomycin and thiostrepton of both aminoacyl-tRNA and factor G binding to ribosomes

J Modolell et al. Proc Natl Acad Sci U S A. 1971 Aug.

Abstract

Siomycin, a peptide antibiotic that interacts with the 50S ribosomal subunit and inhibits binding of factor G, is shown also to inhibit binding of aminoacyl-tRNA; however, it does not impair binding of fMet-tRNA and completion of the initiation complex. Moreover, unlike other inhibitors of aminoacyl-tRNA binding (tetracycline, sparsomycin, and streptogramin A), siomycin completely abolishes the GTPase activity associated with the binding of aminoacyl-tRNA catalyzed by factor T(u). A single-site interaction of siomycin appears to be responsible for its effect on both the binding of the aminoacyl-tRNA-T(u)-GTP complex and that of factor G.

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References

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