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. 1971 Aug;68(8):1828-32.
doi: 10.1073/pnas.68.8.1828.

Mechanism of initiation and repression of in vitro transcription of the lac operon of Escherichia coli

L Eron, R Block

Mechanism of initiation and repression of in vitro transcription of the lac operon of Escherichia coli

L Eron et al. Proc Natl Acad Sci U S A. 1971 Aug.

Abstract

A cyclic AMP-binding protein (CAP protein), cyclic AMP, and RNA polymerase holoenzyme are shown to initiate lac transcription at the lac promoter. Lac repressor appears to control transcription by preventing RNA polymerase and/or CAP protein from binding to the lac promoter. Results support the idea that the lac promoter is composed of two sites that interact with CAP protein and RNA polymerase holoenzyme. The promoter can be altered by mutation so that holoenzyme alone can initiate lac transcription correctly.

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References

    1. Nat New Biol. 1971 Jan 20;229(3):69-74 - PubMed
    1. Nature. 1969 Mar 15;221(5185):1012-4 - PubMed
    1. Nature. 1969 Dec 20;224(5225):1168-74 - PubMed
    1. Nat New Biol. 1971 Mar 17;230(11):76-80 - PubMed
    1. J Mol Biol. 1969 Feb 28;40(1):145-51 - PubMed

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