doi: 10.1073/pnas.68.12.2913.
Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae
- PMID: 4332003
- PMCID: PMC389558
- DOI: 10.1073/pnas.68.12.2913
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Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae
Proc Natl Acad Sci U S A.
1971 Dec.
Abstract
A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 x 10(5) to 7.4 x 10(4) daltons, as determined by electron microscopy, DNA content, or electrophoretic mobility.
Comment in
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Danna and Nathans: Restriction enzymes and the boon to modern molecular biology.Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):5909. doi: 10.1073/pnas.0502760102. Epub 2005 Apr 19. Proc Natl Acad Sci U S A. 2005. PMID: 15840710 Free PMC article. No abstract available.
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How restriction enzymes became the workhorses of molecular biology.Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):5905-8. doi: 10.1073/pnas.0500923102. Epub 2005 Apr 19. Proc Natl Acad Sci U S A. 2005. PMID: 15840723 Free PMC article.
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