Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 Dec;68(12):2913-7.
doi: 10.1073/pnas.68.12.2913.

Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae

K Danna, D Nathans

Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae

K Danna et al. Proc Natl Acad Sci U S A. 1971 Dec.

Abstract

A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 x 10(5) to 7.4 x 10(4) daltons, as determined by electron microscopy, DNA content, or electrophoretic mobility.

PubMed Disclaimer

Comment in

References

    1. Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 - PubMed
    1. Biophys J. 1963 Jul;3:309-21 - PubMed
    1. Anal Biochem. 1965 May;11(2):389-91 - PubMed
    1. Biochem Biophys Res Commun. 1965 Aug 16;20(4):393-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1965 May;53(5):1104-11 - PubMed

MeSH terms

LinkOut - more resources