doi: 10.1128/am.22.5.925-927.1971.
Preparation of type 2 herpes simplex virus complement-fixing antigen
- PMID: 4332046
- PMCID: PMC376448
- DOI: 10.1128/am.22.5.925-927.1971
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Preparation of type 2 herpes simplex virus complement-fixing antigen
Appl Microbiol.
1971 Nov.
Abstract
Comparable complement-fixing antigens of type 1 and type 2 herpes simplex virus were produced by extraction of infected African green monkey cells with 0.85% NaCl which was buffered at pH 9.0 with 0.05 m glycine-NaOH. The optimal antigen dilutions were higher in titrations against hyperimmune animal sera than in titrations against human sera. Complement-fixing antibody to type 2 herpes antigen was detected in 5 of 17 sera from healthy humans.
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