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. 1971 Nov;22(5):925-7.
doi: 10.1128/am.22.5.925-927.1971.

Preparation of type 2 herpes simplex virus complement-fixing antigen

E L Palmer, M L Martin, D T Warfield

Preparation of type 2 herpes simplex virus complement-fixing antigen

E L Palmer et al. Appl Microbiol. 1971 Nov.

Abstract

Comparable complement-fixing antigens of type 1 and type 2 herpes simplex virus were produced by extraction of infected African green monkey cells with 0.85% NaCl which was buffered at pH 9.0 with 0.05 m glycine-NaOH. The optimal antigen dilutions were higher in titrations against hyperimmune animal sera than in titrations against human sera. Complement-fixing antibody to type 2 herpes antigen was detected in 5 of 17 sera from healthy humans.

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References

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