Teichoic acid hydrolase activity in soil bacteria (Bacillus subtilis-sporulation-phosphodiesterase-polyamines-concanavalin A)

Abstract

Bacterial phosphodiesterases have been found that are capable of cleaving the backbone of teichoic acid. Such enzymes have not been reported previously. An aerobic, gram-negative, rod-shaped bacterium producing this activity was detected and isolated by autoradiography of soil suspensions growing on minimal medium containing (32)P-labeled Bacillus subtilis ATCC 6051 cell walls as the sole phosphorus source. Broken-cell preparations are capable of depolymerizing teichoic acids in media of low ionic strength at near-neutral pH values. An active teichoicase is also present in B. subtilis, ATCC 6051 (the Marburg strain), especially in sporulating cultures.