Mutants of Escherichia coli K-12 blocked in the final reaction of ubiquinone biosynthesis: characterization and genetic analysis
- PMID: 4333375
- PMCID: PMC247260
- DOI: 10.1128/jb.109.1.134-139.1972
Mutants of Escherichia coli K-12 blocked in the final reaction of ubiquinone biosynthesis: characterization and genetic analysis
Abstract
The ubiquinone precursor 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1, 4-benzoquinone was isolated from two ubiquinone-deficient mutants of Escherichia coli and identified by nuclear magnetic resonance and mass spectrometry. The results of genetic analysis of the mutants indicate that each mutant carries a mutation in a gene designated ubiG which was located, by cotransduction with the nalA and glpT genes, at minute 42 on the E. coli chromosome.
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