Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1972 Jan;9(1):130-42.
doi: 10.1128/JVI.9.1.130-142.1972.

RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction

J P Leis, J Hurwitz

RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction

J P Leis et al. J Virol. 1972 Jan.

Abstract

The role of ribonucleic acid (RNA) in deoxyribonucleic acid (DNA) synthesis with the purified DNA polymerase from the avian myeloblastosis virus has been studied. The polymerase catalyzes the synthesis of DNA in the presence of four deoxynucleoside triphosphates, Mg(2+), and a variety of RNA templates including those isolated from avian myeloblastosis, Rous sarcoma, and Rauscher leukemia viruses; phages f2, MS2, and Qbeta; and synthetic homopolymers such as polyadenylate.polyuridylic acid. The enzyme does not initiate the synthesis of new chains but incorporates deoxynucleotides at 3' hydroxyl ends of primer strands. The product is an RNA.DNA hybrid in which the two polynucleotide components are covalently linked. Free DNA has not been detected among the products formed with the purified enzyme in vitro. The DNA synthesized with avian myeloblastosis virus RNA after alkaline hydrolysis has a sedimentation coefficient of 6 to 7S.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1965 Jul;54(1):137-44 - PubMed
    1. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1686-92 - PubMed
    1. Proc Natl Acad Sci U S A. 1966 Jan;55(1):219-27 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 Mar;59(3):930-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1511-8 - PubMed

MeSH terms

LinkOut - more resources