Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1972 May;9(5):842-50.
doi: 10.1128/JVI.9.5.842-850.1972.

Solubilization of envelopes of HVJ (Sendai virus) with alkali-emasol treatment and reassembly of envelope particles with removal of the detergent

K Shimizu, Y Hosaka, Y K Shimizu

Solubilization of envelopes of HVJ (Sendai virus) with alkali-emasol treatment and reassembly of envelope particles with removal of the detergent

K Shimizu et al. J Virol. 1972 May.

Abstract

The envelopes of HVJ (Sendai virus) virions were solubilized with alkali-Emasol treatment. The solubilized envelope subunit(s) associated with hemagglutination-inhibiting antibody blocking, neuraminidase, and low hemagglutinating (HA) activities had a sedimentation coefficient of 8.8S. Envelope fragment-like structures were assembled from the solubilized subunits after Emasol was removed by gel filtration. These reassembled envelope particles with HA activity had cell-fusion activity as well as hemolytic activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the reassembled particles revealed that they mainly consisted of two kinds of polypeptides.

PubMed Disclaimer

References

    1. J Biol Chem. 1961 May;236:1372-9 - PubMed
    1. Exp Cell Res. 1965 Oct;40(1):154-8 - PubMed
    1. Biochemistry. 1964 May;3:723-30 - PubMed
    1. Virology. 1970 Mar;40(3):643-54 - PubMed
    1. Virology. 1969 May;38(1):105-19 - PubMed

MeSH terms

LinkOut - more resources