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. 1972 Jun;12(6):625-35.
doi: 10.1016/S0006-3495(72)86108-3.

Manganous ion as a spin label in studies of mitochondrial uptake of manganese

T E Gunter, J S Puskin

Manganous ion as a spin label in studies of mitochondrial uptake of manganese

T E Gunter et al. Biophys J. 1972 Jun.

Abstract

Manganous ion (Mn(2+)) has been used as a spin label for studies of divalent cation uptake by rat liver mitochondria. Spin exchange, observed in the electron paramagnetic resonance (EPR) spectrum of a fraction of the transported Mn(2+), shows that this fraction is bound in regions of high local concentration within the mitochondria. The average separation of manganese ions in that fraction is estimated to be 4.0 +/-0.6 A at the time of greatest concentration.

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