Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1972 Aug;10(2):288-96.
doi: 10.1128/JVI.10.2.288-296.1972.

Rescue of Epstein-Barr virus from somatic cell hybrids of Burkitt lymphoblastoid cells

R Glaser, F Rapp

Rescue of Epstein-Barr virus from somatic cell hybrids of Burkitt lymphoblastoid cells

R Glaser et al. J Virol. 1972 Aug.

Abstract

A Burkitt lymphoblastoid cell line, P3J-HR-1, was fused and hybridized to a human sternal marrow cell line. The somatic cell hybrids were negative when examined for Epstein-Barr virus (EBV) markers. When the hybrid cells were exposed to 5-iododeoxyuridine, both EBV-specific antigens and virus particles were induced as determined by the immunofluorescence test and by electron microscopy. The data presented suggest that the EBV genome can be transferred from a lymphoblastoid cell to another cell type during cell hybridization, that the EBV genome can persist in the hybrid cells for long periods of time, and that synthesis of the virus can be induced in the heterokaryons.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1972 Jan;69(1):83-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1970 May;66(1):79-86 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 Jul;58(1):127-33 - PubMed
    1. J Exp Med. 1965 May 1;121:761-70 - PubMed
    1. J Bacteriol. 1966 Mar;91(3):1248-56 - PubMed

MeSH terms