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. 1972 Oct;6(4):508-12.
doi: 10.1128/iai.6.4.508-512.1972.

Immunization of mice against encephalomyocarditis virus. I. Purification, concentration, and inactivation of encephalomyocarditis virus

W J Bogaerts, Durville-van der Oord

Immunization of mice against encephalomyocarditis virus. I. Purification, concentration, and inactivation of encephalomyocarditis virus

W J Bogaerts et al. Infect Immun. 1972 Oct.

Abstract

Methods are described for the concentration, purification, and inactivation of encephalomyocarditis virus grown in L-cell suspension cultures. Virus concentrates were obtained by the precipitation of crude virus lysates with polyethylene glycol and were purified by Genetron extraction. After column chromatography approximately 70% recovery of infectivity and hemagglutination (HA) activity was found. Exposure of the purified virus suspensions to ultraviolet light resulted in reduction of the infectivity by a factor of 10(9) with little or no loss of HA activity. The preparations are being evaluated as vaccines.

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