An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes
- PMID: 4345164
- PMCID: PMC2108827
- DOI: 10.1083/jcb.56.1.191
An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes
Abstract
A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is suitable for studies of ribosome-membrane interaction.
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