Dibutyryl cyclic adenosine 3'5' monophosphate, sugar transport, and regulatory control of cell division in normal and transformed cells
- PMID: 4345555
- PMCID: PMC2108898
- DOI: 10.1083/jcb.56.2.487
Dibutyryl cyclic adenosine 3'5' monophosphate, sugar transport, and regulatory control of cell division in normal and transformed cells
Abstract
Swiss 3T3 cells exhibit contact-regulated cell growth and have a lower ability to transport 2-deoxyglucose than polyoma (Py)-transformed 3T3 cells. Py3T3 cells treated with dibutyryl cyclic adenosine 3'5' monophosphate (dBcAMP) and theophylline have reduced cell growth and transport 2-deoxyglucose at the same rate as normal 3T3 cells. Evidence that the cessation of cell growth and reduced transport abilities in Py3T3 cells does not represent a return to contact-regulated growth comes from the following observations. First, treating high density Py3T3 cells with dBcAMP allows more than two doublings of cell number, even though ability to transport 2-deoxyglucose is returned to levels equal to those of normal 3T3 cells. Second, dBcAMP prevents serum-stimulated increases in 2-deoxyglucose transport in Py3T3 but not in 3T3 cells.
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