Isolation of an infectious ribonucleoprotein from vesicular stomatitis virus containing an active RNA transcriptase
- PMID: 4347429
- PMCID: PMC355093
- DOI: 10.1128/JVI.11.2.279-286.1973
Isolation of an infectious ribonucleoprotein from vesicular stomatitis virus containing an active RNA transcriptase
Abstract
A ribonucleoprotein complex (TNP) containing an active RNA polymerase was isolated from purified vesicular stomatitis virus particles. The TNP sedimented through a sucrose gradient as a single band and appeared under the electron microscope as discrete long filaments in a spiral configuration. TNP contained one major and two minor polypeptides, but not the polypeptides associated with the outer coat of vesicular stomatitis virus. BHK-21 clone 13 cells could be infected with TNP, yielding infectious virus particles.
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