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. 1973 Jun 1;180(4089):968-71.
doi: 10.1126/science.180.4089.968.

Radioreceptor assay for prolactin and other lactogenic hormones

R P Shiu, P A Kelly, H G Friesen

Radioreceptor assay for prolactin and other lactogenic hormones

R P Shiu et al. Science. .

Abstract

A radioreceptor assay with a sensitivity of 5 nanograms per milliliter has been developed for mammalian and avian pituitary prolactin, placental lactogenic hormones, and humnan growth hormone, using a membrane receptor preparation isolated from rabbit mammary glands. Prolactin preparations inhibited the binding of [(125)I]prolactin to receptors in direct proportion to the biological potency of these preparations. Thus, the radioreceptor assay provides a convenient and simnple assay for hormones which have lactogenic activity.

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