Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1973 May;11(5):615-20.
doi: 10.1128/JVI.11.5.615-620.1973.

Molecular weight determination of Sendai RNA by dimethyl sulfoxide gradient sedimentation

D Kolakofsky, A Bruschi

Molecular weight determination of Sendai RNA by dimethyl sulfoxide gradient sedimentation

D Kolakofsky et al. J Virol. 1973 May.

Abstract

The molecular weight of the large RNA of Sendai virus has been determined by sedimentation analysis in sucrose gradients containing 99% dimethyl sulfoxide (DMSO) to be 2.3 x 10(6). Sendai RNA recovered from 99% DMSO was found to cosediment with nondenatured Sendai RNA at 46 to 48s in ordinary sucrose gradients. The molecular weight value of 2.3 x 10(6) is considerably smaller than the estimates of 6 x 10(6) to 7 x 10(6) determined under nondenaturing conditions, suggesting a unique structure for Sendai RNA.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1965 Sep;54(3):794-800 - PubMed
    1. Virology. 1966 Jun;29(2):205-21 - PubMed
    1. J Mol Biol. 1966 Jun;18(1):195-203 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 Apr;57(4):949-56 - PubMed
    1. Virology. 1967 Oct;33(2):344-6 - PubMed

MeSH terms

LinkOut - more resources