Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1973 Apr;70(4):1037-40.
doi: 10.1073/pnas.70.4.1037.

Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent

J D Sipe, J de Maeyer-Guignard, B Fauconnier, E de Maeyer

Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent

J D Sipe et al. Proc Natl Acad Sci U S A. 1973 Apr.

Abstract

A solid-phase immunoadsorbent capable of binding mouse interferon has been prepared. Starting from crude tissue-culture material, interferon could be purified 1990 times in a single step of affinity chromatography. Overall recovery ranged from 55 to 103% with tissue culture and mouse-brain interferon; however, only 5% was obtained with Sendai virus-induced interferon from mouse serum.

PubMed Disclaimer

References

    1. Nature. 1967 Sep 30;215(5109):1491-2 - PubMed
    1. J Biol Chem. 1970 Jun;245(12):3059-65 - PubMed
    1. J Natl Cancer Inst. 1970 Aug;45(2):365-76 - PubMed
    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Arch Gesamte Virusforsch. 1971;34(3):232-43 - PubMed

LinkOut - more resources