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. 1979 Feb 1;177(2):693-5.
doi: 10.1042/bj1770693.

Removal of cadmium(II) from crystallized ferritin

J Hegenauer, P Saltman, L Hatlen

Removal of cadmium(II) from crystallized ferritin

J Hegenauer et al. Biochem J. .

Abstract

The cadmium content of crystallized horse spleen ferritin, usually about 2% by weight without special treatment, can be substantially decreased by prolonged dialysis against certain chelating agents, chaotropic ions, or weakly reducing anions. For example, neutral bisulphite buffer (2M) removed 95% of the bound cadmium of crystallization without affecting the iron content, and may thus be valuable for preparing "metal-free" holoferritin for physical-chemical studies.

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