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. 1973 Aug;12(2):218-25.
doi: 10.1128/JVI.12.2.218-225.1973.

Hydroxyapatite chromatography and formamide denaturation of adenovirus DNA

C Tibbetts, K Johansson, L Philipson

Hydroxyapatite chromatography and formamide denaturation of adenovirus DNA

C Tibbetts et al. J Virol. 1973 Aug.

Abstract

Denatured adenovirus DNA was retained by hydroxyapatite columns under conditions generally used for selective retention of double-stranded DNA, probably due to several partially complementary sequences within single-stranded DNA. It was found that addition of formamide reduced the fraction of sonically treated, denatured adenovirus DNA bound to hydroxyapatite from about 30% to less than 1%. This led to a study of the effect of formamide on the melting temperature (T(m)) of double-stranded DNA in solution or bound to hydroxyapatite. The T(m) of DNA decreases 0.56 C/1% formamide, a value determined in buffered solutions with purified formamide.

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References

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