Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1973 May;7(5):731-4.
doi: 10.1128/iai.7.5.731-734.1973.

Physical separation of streptococcal nicotinamide adenine dinucleotide glycohydrolase from streptolysin O

S Shany, P S Grushoff, A W Bernheimer

Physical separation of streptococcal nicotinamide adenine dinucleotide glycohydrolase from streptolysin O

S Shany et al. Infect Immun. 1973 May.

Abstract

Streptococcal nicotinamide adenine dinucleotide glycohydrolase (NADase) with a molecular weight of about 55,000 and an isoelectric pH of 8.55 was isolated from crude streptolysin O (SLO) preparations. NADase differed from SLO in size, charge, and immunological behavior. Streptococcal NADase is considered to have no role in the hemolytic process because it has no hemolytic activity; conversely, partially purified SLO showed no NADase activity. The hemolytic activity of crude SLO was completely inhibited by anti-tetanolysin, whereas the NADase activity in the same reaction mixture was unaffected. Experiments involving double diffusion in agar also demonstrated immunological nonidentity of the two proteins.

PubMed Disclaimer

References

    1. J Gen Microbiol. 1967 Jan;46(1):143-50 - PubMed
    1. J Bacteriol. 1968 Jun;95(6):2439-41 - PubMed
    1. J Chromatogr. 1969 Apr 22;41(1):43-52 - PubMed
    1. J Biol Chem. 1969 Aug 25;244(16):4406-12 - PubMed
    1. J Bacteriol. 1969 Oct;100(1):526-7 - PubMed

LinkOut - more resources