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. 1973 Nov;12(5):969-83.
doi: 10.1128/JVI.12.5.969-983.1973.

Determination of the molecular weight of animal RNA viral genomes by nuclease digestions. I. Vesicular stomatitis virus and its defective T particle

P Repik, D H Bishop

Determination of the molecular weight of animal RNA viral genomes by nuclease digestions. I. Vesicular stomatitis virus and its defective T particle

P Repik et al. J Virol. 1973 Nov.

Abstract

A procedure has been developed for the determination of the weight of animal RNA virus genomes using controlled nuclease digestions and computation of the moles of oligonucleotides obtained from 1 mol of RNA. Using both pancreatic RNase and RNase T(1) to digest viral RNA labeled by (3)H-uridine, (3)H-cytidine, or (3)H-guanosine, the weight of the virion RNA of vesicular stomatitis virus (VSV) is estimated as 3.82 +/- 0.14 x 10(6) whereas that of the VSV-defective T particle is estimated as 1.23 +/- 0.04 x 10(6).

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References

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