Enzymatic radioiodination of the envelope proteins of avian myeloblastosis virus
- PMID: 4359430
- PMCID: PMC355256
- DOI: 10.1128/JVI.13.1.42-45.1974
Enzymatic radioiodination of the envelope proteins of avian myeloblastosis virus
Abstract
Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mild detergent treatment and subsequent recovery of immune complexes showed this technique to be useful for the isolation of viral envelope antigens.
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