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. 1973 Dec;70(12):3744-8.
doi: 10.1073/pnas.70.12.3744.

Size and base composition of RNA in supercoiled plasmid DNA

H Williams, H W Boyer, D R Helsinki

Size and base composition of RNA in supercoiled plasmid DNA

H Williams et al. Proc Natl Acad Sci U S A. 1973 Dec.

Abstract

The average size and base composition of the covalently integrated RNA segment in supercoiled ColE(1) DNA synthesized in Escherichia coli in the presence of chloramphenicol (CM-ColE(1) DNA) have been determined by two independent methods. The two approaches yielded similar results, indicating that the RNA segment in CM-ColE(1) DNA contains GMP at the 5' end and comprises on the average 25 to 26 ribonucleotides with a base composition of 10-11 G, 3 A, 5-6 C, and 6-7 U.

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References

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