doi: 10.1073/pnas.70.12.3744.
Size and base composition of RNA in supercoiled plasmid DNA
- PMID: 4359488
- PMCID: PMC427319
- DOI: 10.1073/pnas.70.12.3744
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Size and base composition of RNA in supercoiled plasmid DNA
Proc Natl Acad Sci U S A.
1973 Dec.
Abstract
The average size and base composition of the covalently integrated RNA segment in supercoiled ColE(1) DNA synthesized in Escherichia coli in the presence of chloramphenicol (CM-ColE(1) DNA) have been determined by two independent methods. The two approaches yielded similar results, indicating that the RNA segment in CM-ColE(1) DNA contains GMP at the 5' end and comprises on the average 25 to 26 ribonucleotides with a base composition of 10-11 G, 3 A, 5-6 C, and 6-7 U.
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