Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1973 Oct;12(4):766-74.
doi: 10.1128/JVI.12.4.766-774.1973.

Separation of Herpes simplex virus-induced antigens by Concanavalin A affinity chromatography

M Ponce de Leon, H Hessle, G H Cohen

Separation of Herpes simplex virus-induced antigens by Concanavalin A affinity chromatography

M Ponce de Leon et al. J Virol. 1973 Oct.

Abstract

Biologically active herpes simplex virus (HSV)-induced antigens were selectively removed from extracts of infected BHK cells by affinity chromatography by utilizing an insoluble form of concanavalin A (Con A). Soluble extracts of (3)H-glucosamine-labeled, HSV-infected cells were absorbed to a Con A column. Bound material was eluted with alpha-methyl-d-mannoside (alphaMM) and NaCl. The specific activity of the eluted glycoproteins increased by 10-fold. Two broad groups of viral-induced antigens were isolated from Con A. Group I includes two antigens which bind to Con A by a specific mechanism because the antigens are dissociated by alphaMM. Group II contains three antigens which bind to Con A but apparently by a nonspecific or electrolytic mechanism. One antigen in group I was identified as the glycoprotein antigen, CP-1, described previously.

PubMed Disclaimer

References

    1. Arch Biochem Biophys. 1970 Apr;137(2):460-8 - PubMed
    1. J Gen Virol. 1972 Apr;15(1):93-7 - PubMed
    1. Virology. 1970 Apr;40(4):948-60 - PubMed
    1. Arch Biochem Biophys. 1965 Aug;111(2):407-14 - PubMed
    1. Nature. 1960 Oct 8;188:144-5 - PubMed

LinkOut - more resources