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. 1974 Jun;139(3):789-90.
doi: 10.1042/bj1390789.

A spectrophotometric assay of beta-lactamase action on penicillins

S G Waley

A spectrophotometric assay of beta-lactamase action on penicillins

S G Waley. Biochem J. 1974 Jun.

Abstract

A new method for measuring the enzymic hydrolysis of the beta-lactam ring in penicillins is described. The change in extinction in the u.v. region is determined. The method is sensitive (50mum-benzylpenicillin can be used) and convenient.

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