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. 1974 Aug;71(8):3045-9.
doi: 10.1073/pnas.71.8.3045.

The in vitro synthesis of avian myeloblastosis viral RNA sequences

M Jacquet, Y Groner, G Monroy, J Hurwitz

The in vitro synthesis of avian myeloblastosis viral RNA sequences

M Jacquet et al. Proc Natl Acad Sci U S A. 1974 Aug.

Abstract

Isolated nuclei, prepared from myeloblasts of chicks infected with avian myeloblastosis virus, synthesize RNA sequences present in avian myeloblastosis viral RNA. These sequences are also formed during transcription of chromatin, isolated from myeloblasts, by DNA-dependent RNA polymerases purified from Escherichia coli or calfthymus. In the latter case, transcription is alpha-amanitin sensitive. Formation of hybrids between RNA and avian myeloblastosis virus DNA probes has been monitored by the combined use of ribonucleases A, T(1), and H, and ribonucleases specific for single strands.

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References

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