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. 1974 Sep;71(9):3754-7.
doi: 10.1073/pnas.71.9.3754.

Simian virus 40 T antigen binds to DNA

R B Carroll, L Hager, R Dulbecco

Simian virus 40 T antigen binds to DNA

R B Carroll et al. Proc Natl Acad Sci U S A. 1974 Sep.

Abstract

The T antigen of simian virus 40, which may play a role in the control of viral DNA replication, is recovered from nuclei of cells transformed by simian virus 40 in several forms sedimenting at different rates. The large molecular weight forms are converted to the smallest (5 S) form by high salt, suggesting that they differ in the degree of aggregation. All the forms of the antigen bind efficiently to double-stranded DNA-cellulose columns at pH 6.2 and low salt, and elute in two fractions: one at pH 8.0 and low salt, the other at pH 8.0 and high salt. The antigen has little affinity for single-stranded DNA.

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References

    1. Cold Spring Harb Symp Quant Biol. 1968;33:289-305 - PubMed
    1. J Virol. 1969 Nov;4(5):574-9 - PubMed
    1. Fed Proc. 1970 May-Jun;29(3):1154-63 - PubMed
    1. J Virol. 1967 Oct;1(5):1093-5 - PubMed
    1. J Virol. 1971 Feb;7(2):189-97 - PubMed

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