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. 1974 Dec;71(12):4773-7.
doi: 10.1073/pnas.71.12.4773.

Isolation of a viral polypeptide associated with poliovirus RNA polymerase

R E Lundquist, E Ehrenfeld, J V Maizel Jr

Isolation of a viral polypeptide associated with poliovirus RNA polymerase

R E Lundquist et al. Proc Natl Acad Sci U S A. 1974 Dec.

Abstract

Poliovirus-infected HeLa cells were labeled with radioactive methionine or phenylalanine and subjected to a new purification procedure for the viral induced RNA polymerase activity. Detergent-solubilized polymerase activity was purified by precipitation with 2 M LiCl and sedimentation through sucrose gradients. Approximately 0.001% of the incorporated amino acid radio-activity sediments with the peak of polymerase activity. Gradient fractions comprising the polymerase activity peak were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found to contain predominantly one virus-specific polypeptide. Polyacrylamide gel electrophoresis also reveals that this purified polypeptide migrates with a 58,000 molecular weight noncapsid polio-virus polypeptide.

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